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Am J Physiol Regul Integr Comp Physiol (November 23, 2005). doi:10.1152/ajpregu.00727.2005
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Submitted on October 13, 2005
Accepted on November 22, 2005

Differential effects of water and saline intake on water deprivation induced c-Fos staining in the rat

Helmut B Gottlieb1, Lisa L Ji1, Heath Jones1, Maurice L Penny1, Tiffany Fleming1, and J. Thomas Cunningham1*

1 Pharmacology and Center for Biomedical Neuroscience, University of Texas Health Science Center at San Antonio, San Antonio, TX, USA

* To whom correspondence should be addressed. E-mail: cunninghamt{at}uthscsa.edu.

We studied c-Fos staining in adult male rats following 48 h water deprivation and after 46 h water deprivation with 2 h access to either water or physiological saline. Controls were allowed ad libitum access to water and physiological saline. Rats were anesthetized and perfused for immunocytochemistry using a commercially available antibody for c-Fos. Dehydration significantly increased plasma vasopressin, osmolality, plasma renin-activity (PRA), hematocrit, and sodium concentration while decreasing urine volume. Fos staining was significantly increased in the median preoptic nucleus, organum vasculosum of the lamina terminalis, supraoptic nucleus (SON), and magnocellular and parvocellular paraventricular nucleus (PVN), as well as the area postrema, nucleus of the solitary tract (NTS), and the rostral ventrolateral medulla (RVL). Rehydration with water significantly decreased vasopressin levels and Fos staining in the SON, PVN and RVL, while Fos expression in the perinuclear zone of the SON, NTS, and parabrachial nucleus (PBN) significantly increased. Rehydration with water was associated with decreased urine sodium concentration and hypotonicity while hematocrit and PRA were comparable to levels seen after dehydration. Following rehydration with saline, plasma osmolality, hematocrit, and PRA were not different from control but plasma vasopressin and urine sodium concentration were increased. In the SON, Fos staining was significantly increased with a great percentage of the Fos cells also stained for oxytocin compared with water deprivation. Changes in Fos staining were also observed in the NTS, RVL, PBN, and PVN. Rehydration with either water or saline produces differential effects on plasma vasopressin, Fos staining, and sodium concentration.




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