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Am J Physiol Regul Integr Comp Physiol (January 16, 2003). doi:10.1152/ajpregu.00560.2002
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Submitted on September 11, 2002
Accepted on December 17, 2002

Insulin-induced Translocation of Facilitative Glucose Transporters in Fetal/Neonatal Rat Skeletal Muscle

Jing He1, Manikkavasagar Thamotharan2, and Sherin U Devaskar2*

1 Division of Neonatology and Developmental Biology, Department of Pediatrics, University of Pittsburgh School of Medicine, Magee-Womens Research Institute, Pittsburgh, PA 15213-3180, USA
2 Division of Neonatology and Developmental Biology, Department of Pediatrics, David Geffen School of Medicine at UCLA, Los Angeles, CA 90095-1752, USA

* To whom correspondence should be addressed. E-mail: sdevaskar{at}mednet.ucla.edu.

We examined the effect of insulin on fetal/neonatal rat skeletal muscle GLUT 1 and GLUT 4 concentrations and subcellular distribution by employing immunohistochemical analysis and subcellular fractionation followed by Western blot analysis. We observed that insulin did not alter total GLUT 1 or GLUT 4 concentrations, and the GLUT 1 subcellular distribution in fetal/neonatal or adult skeletal muscle in 60 minutes. The basal and insulin-induced changes in subcellular distribution of GLUT 4 were different between the fetal/neonatal and adult skeletal muscle. Under basal conditions, sarcolemma associated GLUT 4 was higher in the newborn when compared to the adult translating into a higher glucose transport. In contrast, insulin-induced translocation of GLUT 4 to the sarcolemma and insulin induced glucose transport were lower in the newborn when compared to the adult. This age-related change results in enhanced basal glucose transport to fuel myocytic proliferation and differentiation while relatively curbing the insulin dependent glucose transport in the newborn.




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