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Am J Physiol Regul Integr Comp Physiol (June 12, 2003). doi:10.1152/ajpregu.00419.2002
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Submitted on July 16, 2002
Accepted on June 5, 2003

Subcellular distribution of ryanodine receptors in the cardiac muscle of carp(Cyprinus carpio)

Akihito Chugun1*, Kazumi Taniguchi2, Takashi Murayama3, Tsuyoshi Uchide4, Yukio Hara5, Kyosuke Temma4, Yasuo Ogawa3, and Tai Akera6

1 Veterinary Pharmacology, Kitasato University, Towada-shi, Aomori, Japan; Pharmacology, Juntendo University, Bunkyo-ku, Tokyo, Japan
2 Veterinary Anatomy, Kitasato University, Towada-shi, Aomori, Japan
3 Pharmacology, Juntendo University, Bunkyo-ku, Tokyo, Japan
4 Toxicology, Kitasato University, Towada-shi, Aomori, Japan
5 Veterinary Pharmacology, Kitasato University, Towada-shi, Aomori, Japan
6 MSDRL, Minato-ku, Tokyo, Japan

* To whom correspondence should be addressed. E-mail: chugun{at}med.juntendo.ac.jp.

We examined the subcellular localization of ryanodine receptors (RyR) in the cardiac muscle of carp using biochemical, immunohistochemical, and electron microscopic methods, and compared it with those of rats and guinea pigs. To achieve this goal, an anti-RyR antibody was newly raised against a synthetic peptide corresponding to an amino acid sequence that was conserved among all sequenced RyRs. Western blot analysis using this antibody detected a single RyR band following the SDS-PAGE of sarcoplasmic reticulum (SR) membranes from carp atrium and ventricle as well as from mammalian hearts and skeletal muscles. The carp heart band had slightly greater mobility than those of mammalian hearts. Although immunohistochemical staining showed evident striations corresponding to the Z-lines in longitudinal sections of mammalian hearts, clusters of punctate staining, in contrast, were distributed ubiquitously throughout carp atrium and ventricle. Electron microscopic images of the carp myocardium showed that the SR was observed largely as the subsarcolemmal cisternae and the reticular SR, suggesting that the RyR is localized in the junctional and corbular SR.




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