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TRANSLATIONAL PHYSIOLOGY

Mechanisms of impaired calcium handling underlying subclinical diastolic dysfunction in diabetes

¹College of Pharmacy, ²Dorothy M. Davis Heart and Lung Research Institute, and ³Departments of Physiology and Cell Biology, ⁴Internal Medicine, and ⁵Veterinary Clinical Sciences, The Ohio State University, Columbus, Ohio

Submitted 25 January 2007 ; accepted in final form 24 August 2007

ABSTRACT

Isolated diastolic dysfunction is found in almost half of asymptomatic patients with well-controlled diabetes and may precede diastolic heart failure. However, mechanisms that underlie diastolic dysfunction during diabetes are not well understood. We tested the hypothesis that isolated diastolic dysfunction is associated with impaired myocardial Ca²⁺ handling during type 1 diabetes. Streptozotocin-induced diabetic rats were compared with age-matched placebo-treated rats. Global left ventricular myocardial performance and systolic function were preserved in diabetic animals. Diabetes-induced diastolic dysfunction was evident on Doppler flow imaging, based on the altered patterns of mitral inflow and pulmonary venous flows. In isolated ventricular myocytes, diabetes resulted in significant prolongation of action potential duration compared with controls, with afterdepolarizations occurring in diabetic myocytes (P < 0.05). Sustained outward K⁺ current and peak outward component of the inward rectifier were reduced in diabetic myocytes, while transient outward current was increased. There was no significant change in L-type Ca²⁺ current; however, Ca²⁺ transient amplitude was reduced and transient decay was prolonged by 38% in diabetic compared with control myocytes (P < 0.05). Sarcoplasmic reticulum Ca²⁺ load (estimated by measuring the integral of caffeine-evoked Na⁺-Ca²⁺ exchanger current and Ca²⁺ transient amplitudes) was reduced by 50% in diabetic myocytes (P < 0.05). In permeabilized myocytes, Ca²⁺ spark amplitude and frequency were reduced by 34 and 20%, respectively, in diabetic compared with control myocytes (P < 0.05). Sarco(endo)plasmic reticulum Ca²⁺-ATPase-2a protein levels were decreased during diabetes. These data suggest that in vitro impairment of Ca²⁺ reuptake during myocyte relaxation contributes to in vivo diastolic dysfunction, with preserved global systolic function, during diabetes.

diastole; diabetes mellitus; cardiomyopathy; echocardiography; electrophysiology

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