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Physiology and Pharmacology of Temperature Regulation

Are transcription factors NF-B and AP-1 involved in the ANG II-stimulated production of proinflammatory cytokines induced by LPS in dehydrated rats?

¹Division of Integrative Physiology, Department of Functional, Morphological and Regulatory Science and ²Division of Molecular Biology, Department of Molecular Biology, Tottori University Faculty of Medicine, Tottori, Japan

Submitted 18 February 2005 ; accepted in final form 25 July 2005

We recently reported an involvement of ANG II and the ANG II type 1 (AT₁) receptor in the hepatic expression of IL-1 induced in dehydrated rats by LPS. Here, we first confirmed that ANG II and AT₁ receptors contribute to the LPS-induced increase in the splenic concentration of IL-1 in dehydrated rats. We then investigated whether ANG II contributes to IL-1 production through a modulating effect on the activation of proinflammatory transcription factors (NF-B and AP-1) that is induced in the dehydrated rat's liver and spleen by intravenous injection of LPS. Surprisingly, LPS markedly increased the hepatic activation of NF-B, an effect that was significantly enhanced (rather than reduced) by pretreatment with an ANG-converting-enzyme (ACE) inhibitor or AT₁-receptor antagonist. Furthermore, the same ACE inhibitor and AT₁-receptor antagonist each increased the resting NF-B activity in the liver and spleen, although they had no effect on the LPS-induced splenic expression of NF-B. Both hepatic and splenic AP-1 expressions were enhanced by LPS. This response was significantly augmented by pretreatment with the AT₁-receptor antagonist (but not with the ACE inhibitor) in the spleen, while in the liver, neither drug had any effect. These results suggest that the endogenous ANG II or AT₁ receptor suppresses the activation of hepatic or splenic transcription factors in dehydrated rats given LPS. Our results seem not to support the idea that NF-B and AP-1 play key roles in the ANG II-induced enhancement of the production of proinflammatory cytokines that is induced by LPS in dehydrated rats.

interleukin-1; electrophoretic mobility shift assay; fever

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