HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (9) Citing Articles via Google Scholar Google Scholar Articles by von Meyenburg, C. Articles by Langhans, W. Search for Related Content PubMed PubMed Citation Articles by von Meyenburg, C. Articles by Langhans, W.

INFLAMMATION AND CYTOKINES

Role for CD14, TLR2, and TLR4 in bacterial product-induced anorexia

¹Institute of Animal Sciences, Swiss Federal Institute of Technology, 8603 Schwerzenbach; ³Department of Research, University Hospital, 4031 Basel, Switzerland; and ²Bourne Laboratory, New York Presbyterian Hospital-Weill Cornell Medical College, White Plains, New York 10605

Submitted 14 November 2003 ; accepted in final form 25 March 2004

The cell surface component CD14 and the toll-like receptors 2 and 4 (TLR2 and TLR4) are important in mediating the immune responses to bacterial products in mammals. Using mice genetically deficient in CD14, TLR2, or TLR4, we studied the role of these molecules in the anorectic effects of LPS and muramyl dipeptide (MDP). CD14 or TLR2 knockout (KO) and TLR4-deficient (TLR4-DEF) mice as well as corresponding wild-type (WT) colittermates were injected intraperitoneally at dark onset with LPS (2 µg/mouse), MDP (10 mg/kg), interleukin-1 (IL-1, 150 ng/mouse), or vehicle, and food intake was recorded. LPS and MDP reduced food intake in WT mice of all genotypes tested. The anorectic effect of LPS was attenuated (P < 0.04) in CD14-KO and TLR4-DEF mice but not in TLR2-KO (P > 0.05). The anorectic effect of MDP was blunted in CD14-KO and TLR2-KO (P < 0.02) mice but not in TLR4-DEF mice. IL-1 reduced food intake similarly in all genotypes tested. These results indicate that CD14 is involved in mediating the anorectic effects of both LPS and MDP. Furthermore, TLR4 and TLR2 are specifically involved in mediating the anorectic effects of LPS and MDP, respectively. The results are consistent with the hypothesis that TLR4 functions as the true LPS receptor and that TLR2 is involved in recognition of gram-positive bacterial products.

toll-like receptor 2 and 4; food intake; lipopolysaccharide; muramyl dipeptide; cytokines; interleukin-1

This article has been cited by other articles:

				T. Hayashi, H. B. Cottam, M. Chan, G. Jin, R. I. Tawatao, B. Crain, L. Ronacher, K. Messer, D. A. Carson, and M. Corr Mast cell-dependent anorexia and hypothermia induced by mucosal activation of Toll-like receptor 7 Am J Physiol Regulatory Integrative Comp Physiol, July 1, 2008; 295(1): R123 - R132. [Abstract] [Full Text] [PDF]

				T. Hubschle, J. Mutze, P. F. Muhlradt, S. Korte, R. Gerstberger, and J. Roth Pyrexia, anorexia, adipsia, and depressed motor activity in rats during systemic inflammation induced by the Toll-like receptors-2 and -6 agonists MALP-2 and FSL-1 Am J Physiol Regulatory Integrative Comp Physiol, January 1, 2006; 290(1): R180 - R187. [Abstract] [Full Text] [PDF]

				A. A. Steiner, S. Chakravarty, J. R. Robbins, A. S. Dragic, J. Pan, M. Herkenham, and A. A. Romanovsky Thermoregulatory responses of rats to conventional preparations of lipopolysaccharide are caused by lipopolysaccharide per se-- not by lipoprotein contaminants Am J Physiol Regulatory Integrative Comp Physiol, August 1, 2005; 289(2): R348 - R352. [Abstract] [Full Text] [PDF]

				A. A. Romanovsky Anorexia: the toll for lipopolysaccharide recognition Am J Physiol Regulatory Integrative Comp Physiol, August 1, 2004; 287(2): R274 - R275. [Full Text] [PDF]