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Am J Physiol Regul Integr Comp Physiol 279: R1695-R1700, 2000;
0363-6119/00 $5.00
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Vol. 279, Issue 5, R1695-R1700, November 2000

Effect of locally applied drugs on the pH of luminal fluid in the endolymphatic sac of guinea pig

Vincent Couloigner1, Marie Teixeira1, Philippe Hulin2, Olivier Sterkers1, Maurice Bichara1, Brigitte Escoubet1, Gabrielle Planelles2, and Evelyne Ferrary1

1 Institut National de la Santé et de la Recherche Médicale U.426, Faculté Xavier Bichat, Université Paris 7, 75870 Paris Cedex 18; and 2 Institut National de la Santé et de la Recherche Médicale U.467, Faculté Necker Enfants-Malades, Université Paris 5, 75730 Paris Cedex 15, France

The aim of the present work was to assess the effect of various drugs applied locally on the pH of the luminal fluid (pHlum) in guinea pig endolymphatic sac. pHlum and transepithelial potential, when measured in vivo by means of double-barrelled pH-sensitive microelectrodes, were 7.06 ± 0.08 and +6.1 ± 0.34 mV (mean ± SE; n = 84), respectively, which is consistent with a net acid secretion in the luminal fluid of the endolymphatic sac. Bafilomycin and acetazolamide increased and decreased, respectively, pHlum. Amiloride, ethylisopropylamiloride, ouabain, and Schering 28080 had no effect on pHlum. Results obtained with inhibitors of anionic transport systems were inconclusive; e.g., DIDS reduced pHlum, whereas neither SITS nor triflocin had any effect. We conclude that bafilomycin-sensitive H+-ATPase activity accounts for the transepithelial acid gradient measured in the endolymphatic sac and that intracellular and membrane-bound carbonic anhydrase probably participates in regulating endolymphatic sac pHlum. The relationship between acid pH, endolymph volume, and Ménière's disease remains to be further investigated.

acid-base; endolymph; inner ear; H+-ATPase; Ménière's disease


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