AJP - Regu AJP: Gastrointestinal and Liver Physiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Regul Integr Comp Physiol 279: R1580-R1589, 2000;
0363-6119/00 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (22)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Petronini, P.-G.
Right arrow Articles by Wheeler, K. P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Petronini, P.-G.
Right arrow Articles by Wheeler, K. P.
Vol. 279, Issue 5, R1580-R1589, November 2000

Induction of BGT-1 and amino acid System A transport activities in endothelial cells exposed to hyperosmolarity

Pier-Giorgio Petronini1, Roberta R. Alfieri1, M. Nadia Losio2, Alessandro E. Caccamo1, Andrea Cavazzoni1, Mara A. Bonelli1, Angelo F. Borghetti1, and Kenneth P. Wheeler3

1 Dipartimento di Medicina Sperimentale, Sezione di Patologia Molecolare e Immunologia, Università degli Studi di Parma, 43100 Parma; 2 Centro Substrati Cellulari, Istituto Zooprofilattico Sperimentale, 25125 Brescia, Italy; and 3 School of Biological Sciences, University of Sussex, Brighton BN1 9QG, United Kingdom

We studied the responses to hypertonicity of cultured endothelial cells from swine pulmonary arteries. In 0.5 osmol/kgH2O medium, initial cell shrinkage was followed by a regulatory volume increase (RVI), complete after 1 h, concomitant with an increase in cellular K+ content. Then the activity of amino acid transport System A increased, accompanied by an accumulation of ninhydrin-positive solutes (NPS), reaching a peak at ~6 h. The subsequent decline in System A activity was paralleled by an induction of the betaine-GABA transporter (BGT-1), detected as increases of BGT-1 mRNA and of transport activity, which peaked at ~24 h. Inhibitors of transcription or translation prevented induction of both transport activities. The increased expression of BGT-1, which involved activation of "tonicity-responsive enhancer," was inhibited by 5 mM extracellular betaine. Cellular K+ concentration gradually declined after the accumulation of NPS and during the induction of BGT-1. This very effective adaptation to hypertonicity suggests it has a physiological role.

membrane transport; osmolyte; regulatory volume increase; volume


This article has been cited by other articles:


Home page
 J. Physiol.Home page
R. R. Alfieri, M. A. Bonelli, A. Cavazzoni, M. Brigotti, C. Fumarola, P. Sestili, P. Mozzoni, G. De Palma, A. Mutti, D. Carnicelli, et al.
Creatine as a compatible osmolyte in muscle cells exposed to hypertonic stress
J. Physiol., October 15, 2006; 576(2): 391 - 401.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
Q. Cai, J. D. Ferraris, and M. B. Burg
High NaCl increases TonEBP/OREBP mRNA and protein by stabilizing its mRNA
Am J Physiol Renal Physiol, October 1, 2005; 289(4): F803 - F807.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
S. A. Kempson, V. Parikh, L. Xi, S. Chu, and M. H. Montrose
Subcellular redistribution of the renal betaine transporter during hypertonic stress
Am J Physiol Cell Physiol, November 1, 2003; 285(5): C1091 - C1100.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
G. E. Mann, D. L. Yudilevich, and L. Sobrevia
Regulation of Amino Acid and Glucose Transporters in Endothelial and Smooth Muscle Cells
Physiol Rev, January 1, 2003; 83(1): 183 - 252.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online