AJP - Regu Journal of Applied Physiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Regul Integr Comp Physiol 279: R522-R530, 2000;
0363-6119/00 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (8)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Norwood, V. F.
Right arrow Articles by Gomez, R. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Norwood, V. F.
Right arrow Articles by Gomez, R. A.
Vol. 279, Issue 2, R522-R530, August 2000

Novel expression and regulation of the renin-angiotensin system in metanephric organ culture

Victoria F. Norwood, Marjorie Garmey, Jeffrey Wolford, Robert M. Carey, and R. Ariel Gomez

University of Virginia, Children's Medical Center, Charlottesville, Virginia 22908

To evaluate the presence and regulation of the renin-angiotensin system (RAS) in metanephric organ culture, embryonic day 14 (E14) rat metanephroi were cultured for 6 days. mRNAs for renin and both ANG II receptors (AT1 and AT2) are expressed at E14, and all three genes continue to be expressed in culture. Renin mRNA is localized to developing tubules and ureteral branches in the cultured explants. At E14, renin immunostaining is found in isolated cells scattered within the mesenchyme. As differentiation progresses, renin localizes to the ureteric epithelium, developing tubules and glomeruli. E14 metanephroi contain ANG II, and peptide production persists in culture. Renin activity is present at E14 (6.13 ± 0.61 pg ANG I · kidney-1 · h-1) and in cultured explants (28.84 ± 1.13 pg ANG I · kidney-1 · h-1). Renin activity in explants is increased by ANG II treatment (70.1 ± 6.36 vs. 40.97 ± 1.94 pg ANG I · kidney-1 · h-1 in control). This increase is prevented by AT1 blockade, whereas AT2 antagonism has no effect. These studies document an operational local RAS and a previously undescribed positive-feedback mechanism for renin generation in avascular, cultured developing metanephroi. This novel expression pattern and regulatory mechanism highlight the unique ability of developing renal cells to express an active RAS.

angiotensin receptors; kidney development


This article has been cited by other articles:


Home page
 Nephrol Dial TransplantHome page
M. R. Dilworth, M. J. Clancy, D. Marshall, C. A. Bravery, P. E. Brenchley, and N. Ashton
Development and functional capacity of transplanted rat metanephroi
Nephrol. Dial. Transplant., March 1, 2008; 23(3): 871 - 879.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
M. Paul, A. Poyan Mehr, and R. Kreutz
Physiology of local Renin-Angiotensin systems.
Physiol Rev, July 1, 2006; 86(3): 747 - 803.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. GenomicsHome page
H. Castrop, M. Oppermann, Y. Weiss, Y. Huang, D. Mizel, H. Lu, S. Germain, F. Schweda, F. Theilig, S. Bachmann, et al.
Reporter gene recombination in juxtaglomerular granular and collecting duct cells by human renin promoter-Cre recombinase transgene
Physiol Genomics, April 13, 2006; 25(2): 277 - 285.
[Abstract] [Full Text] [PDF]

Home page
 J. Histochem. Cytochem.Home page
M. Loghman-Adham, C. E. Soto, T. Inagami, and C. Sotelo-Avila
Expression of Components of the Renin-angiotensin System in Autosomal Recessive Polycystic Kidney Disease
J. Histochem. Cytochem., August 1, 2005; 53(8): 979 - 988.
[Abstract] [Full Text] [PDF]

Home page
 HypertensionHome page
M. C. Prieto-Carrasquero, L. M. Harrison-Bernard, H. Kobori, Y. Ozawa, K. S. Hering-Smith, L. L. Hamm, and L. G. Navar
Enhancement of Collecting Duct Renin in Angiotensin II-Dependent Hypertensive Rats
Hypertension, August 1, 2004; 44(2): 223 - 229.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
M. L. S. Sequeira Lopez, D. R. Chernavvsky, T. Nomasa, L. Wall, M. Yanagisawa, and R. A. Gomez
The embryo makes red blood cell progenitors in every tissue simultaneously with blood vessel morphogenesis
Am J Physiol Regulatory Integrative Comp Physiol, April 1, 2003; 284(4): R1126 - R1137.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
O. Skott
Renin
Am J Physiol Regulatory Integrative Comp Physiol, April 1, 2002; 282(4): R937 - R939.
[Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online