AJP - Regu AJP: Cell Physiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Regul Integr Comp Physiol 278: R1506-R1512, 2000;
0363-6119/00 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (16)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wu, F.
Right arrow Articles by Mattson, D. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wu, F.
Right arrow Articles by Mattson, D. L.
Vol. 278, Issue 6, R1506-R1512, June 2000

Characterization of L-arginine transporters in rat renal inner medullary collecting duct

Feng Wu, Brian Cholewa, and David L. Mattson

Department of Physiology, Medical College of Wisconsin, Milwaukee, Wisconsin 53226

Previous work from our laboratory has demonstrated that the inner medullary collecting duct (IMCD) expresses a large amount of nitric oxide synthase (NOS) activity. The present study was designed to characterize the transport of NOS substrate, L-arginine, in a suspension of bulk-isolated IMCD cells from the Sprague-Dawley rat kidney. Biochemical transport studies demonstrated an L-arginine transport system in IMCD cells that was saturable and Na+ independent (n = 6). L-Arginine uptake by IMCD cells was inhibited by the cationic amino acids L-lysine, L-homoarginine, and L-ornithine (10 mmol/l each) and unaffected by the neutral amino acids L-leucine, L-serine, and L-glutamine. Both L-ornithine (n = 6) and L-lysine (n = 6) inhibited NOS enzymatic activity in a dose-dependent manner in IMCD cells, supporting the important role of L-arginine transport for NO production by this tubular segment. Furthermore, RT-PCR of microdissected IMCD confirmed the presence of cationic amino acid transporter CAT1 mRNA, whereas CAT2A, CAT2B, and CAT3 were not detected. These results indicate that L-arginine uptake by IMCD cells occurs via system y+, is encoded by CAT1, and may participate in the regulation of NO production in this renal segment.

kidney; kidney collecting duct; nitric oxide synthase


This article has been cited by other articles:


Home page
 Am. J. Physiol. Renal Physiol.Home page
M. Kakoki, H.-S. Kim, C.-J. S. Edgell, N. Maeda, O. Smithies, and D. L. Mattson
Amino acids as modulators of endothelium-derived nitric oxide
Am J Physiol Renal Physiol, August 1, 2006; 291(2): F297 - F304.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
M. Kakoki, H.-S. Kim, W. J. Arendshorst, and D. L. Mattson
L-Arginine uptake affects nitric oxide production and blood flow in the renal medulla
Am J Physiol Regulatory Integrative Comp Physiol, December 1, 2004; 287(6): R1478 - R1485.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
T. Zewde, F. Wu, and D. L. Mattson
Influence of dietary NaCl on L-arginine transport in the renal medulla
Am J Physiol Regulatory Integrative Comp Physiol, January 1, 2004; 286(1): R89 - R93.
[Abstract] [Full Text]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
A. W. Cowley Jr., T. Mori, D. Mattson, and A.-P. Zou
Role of renal NO production in the regulation of medullary blood flow
Am J Physiol Regulatory Integrative Comp Physiol, June 1, 2003; 284(6): R1355 - R1369.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
D. Schwartz, I. F. Schwartz, E. Gnessin, Y. Wollman, T. Chernichovsky, M. Blum, and A. Iaina
Differential regulation of glomerular arginine transporters (CAT-1 and CAT-2) in lipopolysaccharide-treated rats
Am J Physiol Renal Physiol, April 1, 2003; 284(4): F788 - F795.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
T. L. Pallone, Z. Zhang, and K. Rhinehart
Physiology of the renal medullary microcirculation
Am J Physiol Renal Physiol, February 1, 2003; 284(2): F253 - F266.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
F. D. Houghton1, J. A. Hawkhead, P. G. Humpherson, J. E. Hogg, A. H. Balen, A. J. Rutherford, and H. J. Leese
Non-invasive amino acid turnover predicts human embryo developmental capacity
Hum. Reprod., April 1, 2002; 17(4): 999 - 1005.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online