AJP - Regu Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Regul Integr Comp Physiol 278: R781-R789, 2000;
0363-6119/00 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (12)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ozono, R.
Right arrow Articles by Carey, R. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ozono, R.
Right arrow Articles by Carey, R. M.
Vol. 278, Issue 3, R781-R789, March 2000

Expression and localization of angiotensin subtype receptor proteins in the hypertensive rat heart

Ryoji Ozono1, Toshiyuki Matsumoto1, Tetsuji Shingu2, Tetsuya Oshima1, Yasuhiro Teranishi3, Masayuki Kambe1, Hideo Matsuura2, Goro Kajiyama2, Zhi-Qin Wang4, Allan F. Moore4, and Robert M. Carey4

1 Department of Clinical Laboratory Medicine, 2 First Department of Internal Medicine and 3 Second Department of Physiology, Hiroshima University School of Medicine, Hiroshima, Japan 734; and 4 Department of Internal Medicine, University of Virginia Health Sciences Center, Charlottesville, Virginia 22908

The cellular localization of the AT2 receptor and the regulation of its expression in hypertrophied left ventricle are not well known. We compared the expression of the cardiac AT1 and AT2 receptor in spontaneously hypertensive rats/Izumo strain (SHR/Izm) and Wistar Kyoto rats/Izumo strain (WKY/Izm), ages 4, 12, and 20 wk, by means of immunohistochemistry and Western blot analysis. In SHR/Izm, compared with WKY/Izm, blood pressure (161 ± 2 vs. 120 ± 2 mmHg at 12 wk, P <=  0.01, and 199 ± 3 vs. 123 ± 3 mmHg at 20 wk, P <=  0.01) and heart-to-body weight ratio (3.76 ± 0.07 vs. 3.06 ± 0.06 mg/g at 12 wk, P <=  0.01, and 3.90 ± 0.08 vs. 3.01 ± 0.12 mg/g at 20 wk, P <=  0.01) were significantly elevated. There was no difference in these values between the two strains at 4 wk of age. Histologically, 20-wk-old SHR/Izm demonstrated myocardial hypertrophy, a thickening of the smooth muscle layer of the intracardiac arteries, and perivascular fibrosis. By immunohistochemistry, the AT2 receptor was localized to cardiomyocytes and vascular endothelial cells, but not in the vascular smooth muscle cells. No major AT2 receptor signal was observed in perivascular fibrosis at any age in either strain of rats. No difference was detected in this localization between the two strains. By Western blotting, a single 44-kDa band for the AT2 receptor and a single 60-kDa band for the AT1 receptor were detected in ventricles from both strains of rats at all ages. Densitometric analysis demonstrated that the AT2 receptor 44-kDa band was decreased by 20% at 12 wk and 32% at 20 wk (P < 0.01) in SHR/Izm compared with WKY/Izm. The intensity of the AT1 receptor 60-kDa band was increased by 57% in 20-wk-old SHR/Izm compared with WKY/Izm (P < 0.05). There was no significant difference in the intensity of the 44- or 60-kDa bands in 4-wk-old animals of either strain. We demonstrated a decrease in the AT2 receptor and an increase in the AT1 receptor protein with no change in their localizations in hypertrophied left ventricular myocytes of SHR/Izm.

immunhistochemistry; AT2 receptor; spontaneously hypertensive rats


This article has been cited by other articles:


Home page
 Journal of Renin-Angiotensin-Aldosterone SystemHome page
T. Backlund, P. Lakkisto, E. Palojoki, T. Gronholm, A. Saraste, P. Finckenberg, E. Mervaala, I. Tikkanen, and M. Laine
Activation of protective and damaging components of the cardiac renin-angiotensin system after myocardial infarction in experimental diabetes
Journal of Renin-Angiotensin-Aldosterone System, June 1, 2007; 8(2): 66 - 73.
[Abstract] [PDF]

Home page
 HypertensionHome page
V. C. Munk, L. Sanchez de Miguel, M. Petrimpol, N. Butz, A. Banfi, U. Eriksson, L. Hein, R. Humar, and E. J. Battegay
Angiotensin II Induces Angiogenesis in the Hypoxic Adult Mouse Heart In Vitro Through an AT2-B2 Receptor Pathway
Hypertension, May 1, 2007; 49(5): 1178 - 1185.
[Abstract] [Full Text] [PDF]

Home page
 HypertensionHome page
P. M. Abadir, A. Periasamy, R. M. Carey, and H. M. Siragy
Angiotensin II Type 2 Receptor-Bradykinin B2 Receptor Functional Heterodimerization
Hypertension, August 1, 2006; 48(2): 316 - 322.
[Abstract] [Full Text] [PDF]

Home page
 HypertensionHome page
Y. Oishi, R. Ozono, Y. Yano, Y. Teranishi, M. Akishita, M. Horiuchi, T. Oshima, and M. Kambe
Cardioprotective Role of AT2 Receptor in Postinfarction Left Ventricular Remodeling
Hypertension, March 1, 2003; 41(3): 814 - 818.
[Abstract] [Full Text] [PDF]

Home page
 HypertensionHome page
S. Kurisu, R. Ozono, T. Oshima, M. Kambe, T. Ishida, H. Sugino, H. Matsuura, K. Chayama, Y. Teranishi, O. Iba, et al.
Cardiac Angiotensin II Type 2 Receptor Activates the Kinin/NO System and Inhibits Fibrosis
Hypertension, January 1, 2003; 41(1): 99 - 107.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online