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Am J Physiol Regul Integr Comp Physiol 278: R770-R780, 2000;
0363-6119/00 $5.00
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Vol. 278, Issue 3, R770-R780, March 2000

Regulation of atrial natriuretic peptide gene expression in gastric antrum by fasting

William R. Gower Jr.1,2,3,4, Khaled F. Salhab2, Wendy L. Foulis2, Nirmala Pillai2, Jason R. Bundy2, David L. Vesely4,5,6,7, Peter J. Fabri3,8, and John R. Dietz4,7

1 Laboratory, 5 Medicine and 8 Surgery Services, James A. Haley Veterans Hospital, and Departments of 2 Biochemistry and Molecular Biology, 6 Internal Medicine, 7 Physiology and Biophysics, and 3 Surgery, University of South Florida, and 4 University of South Florida Cardiac Hormone Center, Tampa, Florida 33612

Atrial natriuretic peptide (ANP) gene expression was localized in the rat gastric antrum using immunohistochemistry and in situ hybridization to mucosal cells in the lower portion of the antropyloric glands. Colocalization of immunoreactive ANP, long-acting natriuretic peptide, i.e., proANP-(1---30), and serotonin in these cells identified them to be enterochromaffin cells. Fasting for 72 h in 8-mo-old (adult) rats produced a significant (P < 0.05) decrease in the levels of ANP prohormone mRNA, immunoreactive proANP-(1-30) and ANP to ~33% of that of fed rats. Fasting in 1-mo-old rats had no effect on these parameters. Transcripts for natriuretic peptide receptor subtypes NPR-A, NPR-B, and NPR-C were found in both mucosa and muscle tissues of the antrum. ANP, brain natriuretic peptide (BNP), and C-type natriuretic peptide (CNP) stimulated the production of cGMP in antral mucosa in vitro with a potency of ANP > BNP >> CNP, suggesting that these receptors were functional. We conclude that fasting decreases ANP prohormone mRNA and its gene products, long-acting natriuretic peptide, and ANP in the antrum of adult rats.

natriuretic peptide receptors; stomach; enterochromaffin cells; reverse transcription-polymerase chain reaction


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