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Am J Physiol Regul Integr Comp Physiol 277: R1553-R1561, 1999;
0363-6119/99 $5.00
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Vol. 277, Issue 6, R1553-R1561, December 1999

Rainbow trout (Oncorhynchus mykiss) possess two somatostatin mRNAs that are differentially expressed

Craig A. Moore, Jeffrey D. Kittilson, Melissa M. Ehrman, and Mark A. Sheridan

Department of Zoology and Regulatory Biosciences Center, North Dakota State University, Fargo, North Dakota 58105

Previously, we isolated a 624-bp cDNA encoding for a 115-amino acid preprosomatostatin containing [Tyr7,Gly10]-somatostatin (SS)-14 (now designated PPSS-II') obtained from the endocrine pancreas (Brockmann bodies) of rainbow trout. In this study we have characterized a second cDNA obtained from trout pancreas that is 600-bp in length and encodes for a 111-amino acid precursor containing [Tyr7,Gly10]-SS-14 (PPSS-II''). The nucleotide and amino acid identity between the two cDNAs is 82.3 and 80.5%, respectively. Both PPSS-II' and PPSS-II'' mRNA were present in esophagus, pyloric ceca, stomach, upper and lower intestine, and pancreas, whereas only SS-II'' mRNA was present in brain. PPSS-II'' mRNA was more abundant than PPSS-II' mRNA in pancreas, whereas PPSS-II' mRNA was more abundant than PPSS-II'' mRNA in stomach. Fasting increased pancreatic PPSS-II'' mRNA levels but had no effect on the levels of PPSS-II' mRNA. These results indicate the existence of two nonallelic pancreatic SS-II genes that are differentially expressed, both in terms of distribution among tissues and in terms of relative abundance within the tissues.

pancreatic somatostatin-II gene; preprosomatostatin-I; preprosomatostatin-II


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