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Am J Physiol Regul Integr Comp Physiol 276: R1682-R1690, 1999;
0363-6119/99 $5.00
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Vol. 276, Issue 6, R1682-R1690, June 1999

Comparative analysis of NMR and NIRS measurements of intracellular PO2 in human skeletal muscle

Tuan-Khanh Tran1, Napapon Sailasuta2, Ulrike Kreutzer1, Ralph Hurd2, Youngran Chung1, Paul Mole3, Shinya Kuno4, and Thomas Jue1

1 Department of Biological Chemistry, School of Medicine, and 3 Department of Exercise Science, University of California, Davis, 5616; 2 General Electric Medical Systems, Fremont, California 94539; and 4 University of Tsukuba, Tsukuba-shi, Ibiraki-ken, 305-8573 Japan

1H NMR has detected both the deoxygenated proximal histidyl Ndelta H signals of myoglobin (deoxyMb) and deoxygenated Hb (deoxyHb) from human gastrocnemius muscle. Exercising the muscle or pressure cuffing the leg to reduce blood flow elicits the appearance of the deoxyMb signal, which increases in intensity as cellular PO2 decreases. The deoxyMb signal is detected with a 45-s time resolution and reaches a steady-state level within 5 min of pressure cuffing. Its desaturation kinetics match those observed in the near-infrared spectroscopy (NIRS) experiments, implying that the NIRS signals are actually monitoring Mb desaturation. That interpretation is consistent with the signal intensity and desaturation of the deoxyHb proximal histidyl Ndelta H signal from the beta -subunit at 73 parts per million. The experimental results establish the feasibility and methodology to observe the deoxyMb and Hb signals in skeletal muscle, help clarify the origin of the NIRS signal, and set a stage for continuing study of O2 regulation in skeletal muscle.

myoglobin; hemoglobin; exercise; bioenergetics; oxygen; near-infrared spectroscopy


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