| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 Department of Physiology and Pharmacology, James Cook University of North Queensland, Townsville, Queensland, Australia 4811; and 2 Department of Biochemistry, Chang Gung College of Medicine and Technology, Kwei-san, Tao-yuan, Taiwan, Republic of China
Tissue spaces were determined in rat heart, liver, and skeletal muscle in vivo using isotopically labeled [14C]inulin. Tracer was injected into the jugular vein of pentobarbital-anesthetized male Sprague-Dawley rats. After a 30-min equilibration period, a blood sample was taken, and heart, liver, and gastrocnemius muscle were excised and immediately freeze clamped at liquid nitrogen temperatures. The extracellular inulin space was 0.209 ± 0.006 (n = 13), 0.203 ± 0.080 (n = 7), and 0.124 ± 0.006 (SE) ml/g wet wt tissue (n = 8) for heart, liver, and skeletal muscle, respectively. Total tissue water was 0.791 ± 0.005 (n = 9), 0.732 ± 0.002 (n = 9), and 0.755 ± 0.005 ml/g wet wt tissue (n = 10) for heart, liver, and skeletal muscle, respectively. Expressed as a percentage of total tissue water, the intracellular space was 73.6, 72.2, and 83.7% for heart, liver, and skeletal muscle, respectively. With use of 2,3-diphospho-D-glyceric acid as a vascular marker, the interstitial space was calculated by subtracting the counts in tissue due to whole blood from total tissue counts and dividing by plasma counts. The interstitial space was 18.8, 22.4, and 14.5% of total tissue water, with accompanying plasma spaces of 7.7, 5.3, and 1.8% for heart, liver, and gastrocnemius muscle, respectively. The tracer method used in this study provides a quantitative assessment of water distribution in tissues of nonnephrectomized rats that has applications for calculation of tissue ion and metabolite concentrations, gradients, and fluxes under normal and pathophysiological conditions.
extracellular space; inulin; intracellular space; muscle interstitial space; plasma space; 2,3-diphospho-D-glyceric acid
This article has been cited by other articles:
|
|
 |
|
  M. G. Wallis, M. F. Lankford, and S. R. Keller Vasopressin is a physiological substrate for the insulin-regulated aminopeptidase IRAP Am J Physiol Endocrinol Metab, October 1, 2007; 293(4): E1092 - E1102. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Jiang, J. M. Guccione, M. B. Ratcliffe, and E. W. Hsu Transmural heterogeneity of diffusion anisotropy in the sheep myocardium characterized by MR diffusion tensor imaging Am J Physiol Heart Circ Physiol, October 1, 2007; 293(4): H2377 - H2384. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. K. Raja, G. H. Raymer, G. R. Moran, G. Marsh, and R. T. Thompson Changes in tissue water content measured with multiple-frequency bioimpedance and metabolism measured with 31P-MRS during progressive forearm exercise J Appl Physiol, October 1, 2006; 101(4): 1070 - 1075. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. A. J. Adegoke, N. Bedard, H. P. Roest, and S. S. Wing Ubiquitin-conjugating enzyme E214k/HR6B is dispensable for increased protein catabolism in muscle of fasted mice Am J Physiol Endocrinol Metab, September 1, 2002; 283(3): E482 - E489. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. P. Dobson, S. Hitchins, and W. E. Teague Jr. Thermodynamics of the Pyruvate Kinase Reaction and the Reversal of Glycolysis in Heart and Skeletal Muscle J. Biol. Chem., July 19, 2002; 277(30): 27176 - 27182. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. W. Hein, S. H. Platts, K. R. Waitkus-Edwards, L. Kuo, S. A. Mousa, and G. A. Meininger Integrin-binding peptides containing RGD produce coronary arteriolar dilation via cyclooxygenase activation Am J Physiol Heart Circ Physiol, December 1, 2001; 281(6): H2378 - H2384. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. H. Cieslar and G. P. Dobson Force reduction uncoupled from pH and H2PO4- in rat gastrocnemius in vivo with continuous 2-Hz stimulation Am J Physiol Regulatory Integrative Comp Physiol, August 1, 2001; 281(2): R511 - R518. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Hitchins, J. M. Cieslar, and G. P. Dobson 31P NMR quantitation of phosphorus metabolites in rat heart and skeletal muscle in vivo Am J Physiol Heart Circ Physiol, August 1, 2001; 281(2): H882 - H887. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Mingrone, A. Bertuzzi, E. Capristo, A. V. Greco, M. Manco, A. Pietrobelli, S. Salinari, and S. B. Heymsfield Unreliable use of standard muscle hydration value in obesity Am J Physiol Endocrinol Metab, February 1, 2001; 280(2): E365 - E371. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Saab, R. T. Thompson, and G. D. Marsh Effects of exercise on muscle transverse relaxation determined by MR imaging and in vivo relaxometry J Appl Physiol, January 1, 2000; 88(1): 226 - 233. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
