| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
AJP - Regulatory, Integrative and Comparative Physiology, Vol 269, Issue 3 621-R629, Copyright © 1995 by American Physiological Society
ARTICLES |
G. Wu and D. A. Knabe
Department of Animal Science, Texas A & M University, College Station 77843-2471, USA.
Arginine is deficient in porcine colostrum and milk, and yet the piglet has a particularly high requirement for this essential amino acid for rapid postnatal growth. To explain this paradox, arginine synthesis was quantified in enterocytes from newborn (0-day-old) and 2- to 7-day-old suckling pigs. Arginine was found to be synthesized from glutamine in 0- to 7-day-old pig enterocytes, but the rates of arginine synthesis were three- to fourfold greater in 0- to 2-day-old pigs than in 7-day-old pigs. To elucidate the developmental change of the intestinal arginine synthesis, the metabolism of glutamine to citrulline, the conversion of citrulline to arginine, and the activities of the enzymes involved were measured. The rates of metabolism of glutamine to citrulline were 2.5- to 3.5-fold greater in enterocytes from 0- to 2-day-old pigs than in cells from 7-day-old pigs, as were the rates of conversion of citrulline to arginine. The activities of all enzymes that synthesize arginine from glutamine, except pyrroline-5-carboxylate synthase and argininosuccinate lyase (ASL), increased in enterocytes from 2-day-old pigs compared with 0-day-old pigs. The activities of all these enzymes decreased by approximately 75% in 7-day-old pigs compared with 2-day-old pigs. Arginase activity was negligible in enterocytes from 0- to 7-day-old pigs, thus minimizing intestinal hydrolysis of newly synthesized arginine and maximizing the endogenous provision of arginine. The results of this study demonstrate the presence of arginine-synthesizing enzymes and their developmental changes in postnatal pig enterocytes.(ABSTRACT TRUNCATED AT 250 WORDS)
This article has been cited by other articles:
|
|
 |
|
  K. L. Urschel, M. Rafii, P. B. Pencharz, and R. O. Ball A multitracer stable isotope quantification of the effects of arginine intake on whole body arginine metabolism in neonatal piglets Am J Physiol Endocrinol Metab, September 1, 2007; 293(3): E811 - E818. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. T. Johnson, R. A. Harris, P. V. Blair, and R. S. Balaban Functional consequences of mitochondrial proteome heterogeneity Am J Physiol Cell Physiol, February 1, 2007; 292(2): C698 - C707. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. L. Urschel, A. K. Shoveller, P. B. Pencharz, and R. O. Ball Arginine synthesis does not occur during first-pass hepatic metabolism in the neonatal piglet Am J Physiol Endocrinol Metab, June 1, 2005; 288(6): E1244 - E1251. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. L. Wilkinson, R. F. P. Bertolo, J. A. Brunton, A. K. Shoveller, P. B. Pencharz, and R. O. Ball Arginine synthesis is regulated by dietary arginine intake in the enterally fed neonatal piglet Am J Physiol Endocrinol Metab, September 1, 2004; 287(3): E454 - E462. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. J de Jonge, M. M Hallemeesch, K. L Kwikkers, J. M Ruijter, C. de Gier-de Vries, M. A van Roon, A. J Meijer, B. Marescau, P. P De Deyn, N. E. Deutz, et al. Overexpression of arginase I in enterocytes of transgenic mice elicits a selective arginine deficiency and affects skin, muscle, and lymphoid development Am. J. Clinical Nutrition, July 1, 2002; 76(1): 128 - 140. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. L. Gookin, J. M. Rhoads, and R. A. Argenzio Inducible nitric oxide synthase mediates early epithelial repair of porcine ileum Am J Physiol Gastrointest Liver Physiol, July 1, 2002; 283(1): G157 - G168. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Wu, N. E. Flynn, and D. A. Knabe Enhanced intestinal synthesis of polyamines from proline in cortisol-treated piglets Am J Physiol Endocrinol Metab, August 1, 2000; 279(2): E395 - E402. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Wu, C. J. Meininger, K. Kelly, M. Watford, and S. M. Morris Jr. A Cortisol Surge Mediates the Enhanced Expression of Pig Intestinal Pyrroline-5-Carboxylate Synthase during Weaning J. Nutr., August 1, 2000; 130(8): 1914 - 1919. [Abstract] [Full Text]
|
|
|
|
|
|
D. E. Barziza, J. A. Buentello, and D. M. Gatlin III Dietary Arginine Requirement of Juvenile Red Drum (Sciaenops ocellatus) Based on Weight Gain and Feed Efficiency J. Nutr., July 1, 2000; 130(7): 1796 - 1799. [Abstract] [Full Text]
|
|
|
|
|
|
P. J. Reeds, D. G. Burrin, B. Stoll, and F. Jahoor Intestinal Glutamate Metabolism J. Nutr., April 1, 2000; 130(4): 978 - 978. [Abstract] [Full Text]
|
|
|
|
|
|
J. A. Brunton, R. F. P. Bertolo, P. B. Pencharz, and R. O. Ball Proline ameliorates arginine deficiency during enteral but not parenteral feeding in neonatal piglets Am J Physiol Endocrinol Metab, August 1, 1999; 277(2): E223 - E231. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Stoll, D. G. Burrin, J. Henry, H. Yu, F. Jahoor, and P. J. Reeds Substrate oxidation by the portal drained viscera of fed piglets Am J Physiol Endocrinol Metab, July 1, 1999; 277(1): E168 - E175. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. L. Dillon, D. A. Knabe, and G. Wu Lactate inhibits citrulline and arginine synthesis from proline in pig enterocytes Am J Physiol Gastrointest Liver Physiol, May 1, 1999; 276(5): G1079 - G1086. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Wu, T. L. Ott, D. A. Knabe, and F. W. Bazer Amino Acid Composition of the Fetal Pig J. Nutr., May 1, 1999; 129(5): 1031 - 1038. [Abstract] [Full Text]
|
|
|
|
|
|
R. F. P. Bertolo, C. Z. L. Chen, P. B. Pencharz, and R. O. Ball Intestinal Atrophy Has a Greater Impact on Nitrogen Metabolism than Liver By-Pass in Piglets Fed Identical Diets via Gastric, Central Venous or Portal Venous Routes J. Nutr., May 1, 1999; 129(5): 1045 - 1052. [Abstract] [Full Text]
|
|
|
|
|
|
N. E. Flynn, C. J. Meininger, K. Kelly, N. H. Ing, S. M. Morris Jr., and G. Wu Glucocorticoids Mediate the Enhanced Expression of Intestinal Type II Arginase and Argininosuccinate Lyase in Postweaning Pigs J. Nutr., April 1, 1999; 129(4): 799 - 803. [Abstract] [Full Text]
|
|
|
|
 |
|
 C.-a. A. Hu, W.-W. Lin, C. Obie, and D. Valle Molecular Enzymology of Mammalian Delta 1-Pyrroline-5-carboxylate Synthase. ALTERNATIVE SPLICE DONOR UTILIZATION GENERATES ISOFORMS WITH DIFFERENT SENSITIVITY TO ORNITHINE INHIBITION J. Biol. Chem., March 5, 1999; 274(10): 6754 - 6762. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Stoll, J. Henry, P. J. Reeds, H. Yu, F. Jahoor, and D. G. Burrin Catabolism Dominates the First-Pass Intestinal Metabolism of Dietary Essential Amino Acids in Milk Protein-Fed Piglets J. Nutr., March 1, 1998; 128(3): 606 - 614. [Abstract] [Full Text]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
