AJP - Regulatory, Integrative and Comparative Physiology, Vol 268, Issue 5 1121-R1128, Copyright © 1995 by American Physiological Society

ARTICLES

Lactate influx into red blood cells of athletic and nonathletic species

M. S. Skelton, D. E. Kremer, E. W. Smith and L. B. Gladden
Department of Health & Human Performance, Auburn University, Alabama 36849-5323, USA.

Transport of lactate across the erythrocyte membrane proceeds by three distinct pathways: 1) nonionic diffusion of lactic acid, 2) inorganic anion exchange (band 3), and 3) a monocarboxylate-specific (MC) carrier mechanism. This study determined the contributions of these three pathways in the red blood cells (RBCs) of "athletic" and "nonathletic" species. Blood samples were obtained from four male animals of each species: 1) Canis familiaris (dogs), 2) Capra hircus (goats), 3) Equus caballus (horses), and 4) Bos taurus (cattle). Contribution of each pathway to total lactate influx was determined by measuring L-[14C]lactate influx into lactate-depleted control RBCs, p-chloromercuribenzenesulfonic acid (PCMBS)-treated (1 mM) RBCs, and 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid (DIDS)-treated (0.2 mM) RBCs at three lactate concentrations ([La] values 1.6, 8.1, and 41 mM). PCMBS blocked MC transport and DIDS blocked the band 3 pathway. Lactate influx into the RBCs of the athletic species was 4-160 times faster (P < 0.05) than influx into the RBCs of the nonathletic species at 8.1 and 41 mM [La] values. Nonionic diffusion was greater in the RBCs of nonathletic animals (approximately 7-25%) than in the RBCs of athletic animals (approximately 4%). A significantly higher percentage of the total lactate influx occurred via the band 3 system in the RBCs from the nonathletic animals (approximately 56-83%) vs. the RBCs from the athletic animals (approximately 6-7%) at all [La] values.(ABSTRACT TRUNCATED AT 250 WORDS)

This article has been cited by other articles:

				S. Fagnete, C. Philippe, H. Olivier, M.-H. Mona, E.-J. Maryse, and H.-D. Marie-Dominique Faster lactate transport across red blood cell membrane in sickle cell trait carriers J Appl Physiol, February 1, 2006; 100(2): 427 - 432. [Abstract] [Full Text] [PDF]

				R. E. Pattillo and L. B. Gladden Red blood cell lactate transport in sickle disease and sickle cell trait J Appl Physiol, September 1, 2005; 99(3): 822 - 827. [Abstract] [Full Text] [PDF]

				P. Connes, D. Bouix, G. Py, C. Caillaud, P. Kippelen, J.-F. Brun, A. Varray, C. Prefaut, and J. Mercier Does exercise-induced hypoxemia modify lactate influx into erythrocytes and hemorheological parameters in athletes? J Appl Physiol, September 1, 2004; 97(3): 1053 - 1058. [Abstract] [Full Text] [PDF]

				G. C. Henderson, M. A. Horning, S. L. Lehman, E. E. Wolfel, B. C. Bergman, and G. A. Brooks Pyruvate shuttling during rest and exercise before and after endurance training in men J Appl Physiol, July 1, 2004; 97(1): 317 - 325. [Abstract] [Full Text] [PDF]

				P. Connes, C. Caillaud, J. Mercier, D. Bouix, and J. F. Casties Injections of recombinant human erythropoietin increases lactate influx into erythrocytes J Appl Physiol, July 1, 2004; 97(1): 326 - 332. [Abstract] [Full Text] [PDF]

				L. K. Vaihkonen, O. J. Heinonen, S. Hyyppa, M. Nieminen, and A. R. Poso Lactate-transport activity in RBCs of trained and untrained individuals from four racing species Am J Physiol Regulatory Integrative Comp Physiol, July 1, 2001; 281(1): R19 - R24. [Abstract] [Full Text] [PDF]

				C. A. Goresky, G. G. Bach, A. Simard, A. J. Schwab, and A. Bracht Uptake of lactate by the liver: effect of red blood cell carriage Am J Physiol Gastrointest Liver Physiol, May 1, 2000; 278(5): G775 - G788. [Abstract] [Full Text] [PDF]

				L. K. Vaihkonen and A. R. Poso Interindividual variation in total and carrier-mediated lactate influx into red blood cells Am J Physiol Regulatory Integrative Comp Physiol, April 1, 1998; 274(4): R1025 - R1030. [Abstract] [Full Text] [PDF]

				P. F. Speake, C. A. Roberts, and J. S. Gibson Effect of changes in respiratory blood parameters on equine red blood cell K-Cl cotransporter Am J Physiol Cell Physiol, December 1, 1997; 273(6): C1811 - C1818. [Abstract] [Full Text] [PDF]