AJP - Regulatory, Integrative and Comparative Physiology, Vol 264, Issue 4 696-R702, Copyright © 1993 by American Physiological Society

ARTICLES

Decreased perfusion pressure modulates renin and ANG II type 1 receptor gene expression in the rat kidney

A. Tufro-McReddie, R. L. Chevalier, A. D. Everett and R. A. Gomez
Department of Pediatrics, University of Virginia School of Medicine, Charlottesville 22908.

To determine whether decreased perfusion pressure affects the abundance and distribution of renin and its mRNA and the expression of the angiotensin II type 1 (AT1) receptor gene within the kidney, adult male Sprague-Dawley rats were subjected to aortic coarctation proximal to the renal arteries (Coarc, n = 8) and compared with sham-operated rats (Sham, n = 6). Renal renin distribution was determined by immunocytochemistry using a specific polyclonal antibody against rat renin. Renin mRNA was assessed by in situ hybridization to a 35S-labeled oligonucleotide complementary to rat renin mRNA. Kidney AT1 mRNA levels were determined by Northern analysis using a 1,133-base pair rat AT1 cDNA. Femoral arterial blood pressure, measured 24 h after surgery, was lower in Coarc than in Sham rats (75 +/- 5.4 vs. 122 +/- 2.3 mmHg, P < 0.05). Aortic coarctation increased the percent of juxtaglomerular apparatuses (%JGA) containing renin and its mRNA (85 +/- 2.5 and 66 +/- 2.8 vs. 49 +/- 5.3 and 36 +/- 1.7%, Coarc vs. Sham, P < 0.05) and the intensity of hybridization signals (497 +/- 89 vs. 71 +/- 12 grains/JGA, Coarc vs. Sham, P < 0.05). In addition, recruitment of renin gene expressing cells was observed along afferent arterioles in Coarc rats, whereas renin and its mRNA were limited to the JGAs in Sham rats. Renal AT1 receptor gene expression was threefold lower in Coarc than in Sham rats. We conclude that reduction of perfusion pressure after abdominal aortic coarctation acutely enhances renin gene expression and downregulates AT1 receptor gene expression.(ABSTRACT TRUNCATED AT 250 WORDS)

This article has been cited by other articles:

				E. S. Pentz, M. L. S. Sequeira Lopez, M. Cordaillat, and R. A. Gomez Identity of the renin cell is mediated by cAMP and chromatin remodeling: an in vitro model for studying cell recruitment and plasticity Am J Physiol Heart Circ Physiol, February 1, 2008; 294(2): H699 - H707. [Abstract] [Full Text] [PDF]

				V. Sahajpal and N. Ashton Increased glomerular angiotensin II binding in rats exposed to a maternal low protein diet in utero J. Physiol., February 15, 2005; 563(1): 193 - 201. [Abstract] [Full Text] [PDF]

				A. J. Mangrum, R. A. Gomez, and V. F. Norwood Effects of AT1A receptor deletion on blood pressure and sodium excretion during altered dietary salt intake Am J Physiol Renal Physiol, September 1, 2002; 283(3): F447 - F453. [Abstract] [Full Text] [PDF]

				G. Nickenig and D. G. Harrison The AT1-Type Angiotensin Receptor in Oxidative Stress and Atherogenesis: Part II: AT1 Receptor Regulation Circulation, January 29, 2002; 105(4): 530 - 536. [Full Text] [PDF]

				M. J. Ryan, T. A. Black, K. W. Gross, and G. Hajduczok Cyclic mechanical distension regulates renin gene transcription in As4.1 cells Am J Physiol Endocrinol Metab, October 1, 2000; 279(4): E830 - E837. [Abstract] [Full Text] [PDF]

				L. Cassis, A. Laughter, M. Fettinger, S. Akers, R. Speth, G. Burke, V. King, and L. Dwoskin Cold Exposure Regulates the Renin-Angiotensin System J. Pharmacol. Exp. Ther., August 1, 1998; 286(2): 718 - 726. [Abstract] [Full Text]

				K. T. Weber Extracellular Matrix Remodeling in Heart Failure : A Role for De Novo Angiotensin II Generation Circulation, December 2, 1997; 96(11): 4065 - 4082. [Full Text]

				J. Wang, F. M. Perez, and J. C. Rose Developmental Changes in Renin-Containing Cells From the Ovine Fetal Kidney Reproductive Sciences, July 1, 1997; 4(4): 191 - 196. [Abstract] [PDF]

				D. H. Wang and Y. Du Distinct Mechanisms of Upregulation of Type 1A Angiotensin II Receptor Gene Expression in Kidney and Adrenal Gland Hypertension, December 1, 1995; 26(6): 1134 - 1137. [Abstract] [Full Text]

				K. Schricker, H. Scholz, M. Hamann, M. Clozel, B. K. Kramer, and A. Kurtz Role of Endogenous Endothelins in the Renin System of Normal and Two-Kidney, One Clip Rats Hypertension, May 1, 1995; 25(5): 1025 - 1029. [Abstract] [Full Text]

				M. Mai, K. F. Hilgers, J. Wagner, J. F. E. Mann, and H. Geiger Expression of Angiotensin-Converting Enzyme in Renovascular Hypertensive Rat Kidney Hypertension, April 1, 1995; 25(4): 674 - 678. [Abstract] [Full Text]