AJP - Regu Watch the video to see how APS reaches out to developing nations.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Regul Integr Comp Physiol 262: R412-R418, 1992;
0363-6119/92 $5.00
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hazzard, C. E.
Right arrow Articles by Ahearn, G. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hazzard, C. E.
Right arrow Articles by Ahearn, G. A.

AJP - Regulatory, Integrative and Comparative Physiology, Vol 262, Issue 3 412-R418, Copyright © 1992 by American Physiological Society

ARTICLES

Rapid stimulation of intestinal D-glucose transport in teleosts by 17 alpha-methyltestosterone

C. E. Hazzard and G. A. Ahearn
Department of Zoology, University of Hawaii, Manoa, Honolulu 96822.

Effects of 17 alpha-methyltestosterone (MT) on transmural transport of 0.5 mM D-[3H]-glucose across upper intestine of the herbivorous teleost, Oreochromis mossambicus, were examined using modified Ussing chambers containing a physiological saline. Treatment tissues were preincubated with MT on the serosal surface for 30 min, while control tissues were incubated with an equivalent volume of 70% ethanol. Stimulation of mucosal-to-serosal D-glucose flux (Jm----s) by MT was dose dependent with maximal transport enhancement at 15 ng MT/ml saline (215% of control) and inhibition of sugar transfer occurring at 50 ng MT/ml (60% of control). Hormone treatment (15 ng MT/ml) significantly (P less than 0.05) increased net transmural D-glucose flux (Jnet; control = 0.36 +/- 0.084, MT = 0.75 +/- 0.130 nmol.cm-2.min-1) as a result of enhancing Jm----s (control = 0.52 +/- 0.048; MT = 0.91 +/- 0.078 nmol.cm-2.-1) without having a significant effect on the serosal-to-mucosal flux (Js----m). Mucosal addition of 1 mM phloridzin significantly (P less than 0.05) reduced glucose transport Jm----s in the presence and absence of MT, without altering the respective Js----m fluxes. Serosal addition of the hormone led to significant increases in Jm----s and Jnet in less than 20 min. This rapid intestinal response to MT was abolished by serosal addition of 1 mM colchicine, and therefore an increased insertion rate of performed glucose carriers is a suggested mechanism of hormone action.


This article has been cited by other articles:


Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
B. Schroeder, M. R. Dahl, and G. Breves
Duodenal Ca2+ absorption is not stimulated by calcitriol during early postnatal development of pigs
Am J Physiol Gastrointest Liver Physiol, August 1, 1998; 275(2): G305 - G313.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online