AJP - Regulatory, Integrative and Comparative Physiology, Vol 252, Issue 2 353-R360, Copyright © 1987 by American Physiological Society
Proglumide, a cholecystokinin antagonist, increases gastric emptying in rats
G. Shillabeer and J. S. Davison
Injection of cholecystokinin (CCK) reduces food intake and delays gastric
emptying. We have previously shown that endogenous CCK also reduces food
intake. This may be achieved by a delay in gastric emptying. We
investigated the role of CCK in gastric emptying by inhibiting the actions
of CCK released by a meal, using a CCK antagonist, proglumide. We
postulated that inhibition of CCK should induce an increase in gastric
emptying. Gastric emptying was determined in rats by a marker dilution
technique using direct gastric intubation. Proglumide (150 mg/kg)
significantly accelerated emptying of liquid food by 12.8% (P less than
0.005, n = 12) when injected intraperitoneally following a food preload.
Proglumide injected before feeding was ineffective. Oral proglumide, which
inhibited gastrin-stimulated acid secretion, was also ineffective. We
concluded that proglumide increased gastric emptying by acting on a factor
released by the preload, and since proglumide is a specific antagonist,
this factor was probably CCK. Therefore CCK may play a physiological role
in the regulation of gastric emptying.
