AJP - Regulatory, Integrative and Comparative Physiology, Vol 239, Issue 3 265-R269, Copyright © 1980 by American Physiological Society
Hypothermia following injection of 2-deoxy-D-glucose into selected hypothalamic sites
T. Shiraishi and M. Mager
From our previous studies with 2-deoxy-D-glucose (2-DG) an inhibitor of
glucose utilization, we postulated that the resultant intracellular
glucopenia affects central neuronal pathways involved in the control of
peripheral heat production. In this investigation, we have delineated these
thermoregulatory sites by stereotaxically injecting microquantities of 2-DG
into the hypothalamus of the rat and monitoring core temperature (Tre).
After stabilization of Tre at a room temperature of 23 +/- 1 degree C, 20
microgram of 2-DG in 2 microliter was injected into 350- to 400-g rats.
Significant decreases in Tre were noted after injections into the anterior
hypothalamic, ventromedial, and dorsomedial nuclei as well as the lateral
and posterior hypothalamic areas. Mean nadir Tre decreased 1.5 degrees C 1
h after, was significantly depressed 3.5 h after, and returned to basal
values 4 h after administration of 2-DG into the ventral premammillary
nucleus (PMV). Dose-dependent response was observed for injections into the
PMV only. Of a total of 21 sites studied in the anterior and posterior
hypothalamus, the PMV, an area of unknown physiological function, was the
most sensitive to glucose deprivation.
