AJP - Regulatory, Integrative and Comparative Physiology, Vol 238, Issue 1 70-R75, Copyright © 1980 by American Physiological Society
Comparison of effects of standard diuretics and indanone in isolated toad cornea and bladder
J. R. Schnieders and J. H. Ludens
Short-circuit current (SCC) techniques were used to monitor effects of
various diuretic agents on Na+ transport in toad bladder and Cl- transport
in toad cornea. In bladder, various agents from different "classes" of
diuretics inhibited SCC whereas in cornea only "loop diuretics," i.e.,
those with a primary site of action in the ascending limb of the loop of
Henle, inhibited SCC. Classification of the diuretics based on sensitivity
in cornea and bladder revealed that diuretics with a common renal site of
action gravitated into common classification groups. Thus, our studies
suggested that cornea may be a suitable model of the thick ascending limb
of the loop of Henle and that cornea and bladder, studied jointly, may
serve as an in vitro system for predicting potential renal sites of action
of new diuretics. This system, when used to characterize the new diuretic
indanone, revealed that this agent, in all respects, displayed the
characteristics of a loop diuretic.
