AJP - Regulatory, Integrative and Comparative Physiology, Vol 236, Issue 1 48-R56, Copyright © 1979 by American Physiological Society
Evaluation of in vivo brain site perfusion with the push-pull cannula
M. P. Honchar, B. K. Hartman and L. G. Sharpe
The effect of tissue site perfusion with push-pull cannulas on the
integrity of brain tissue was evaluated during in vivo studies in conscious
New Zealand rabbits. The mean rate of perfusion-induced tissue disruption
was 1.09 microgram tissue wet wt/min as estimated from the perfusate levels
of endogenous nervous system specific proteins (S-100 and 14-3-2). The
level of intravenously injected 125I-labeled bovine serum albumin recovered
in perfusate samples indicated that approximately 2.0 nl serum/min
penetrated the vascular barrier into the perfused tissue site. The
appearance of intraventricularly injected solutes in perfusate samples was
subjected to regression analysis.It was demonstrated that nonspecific
variations in the appearance of one substance in the perfusate could be
controlled for by a regression on the levels of another concurrently
recovered inert substance. The experiments demonstrate that tissue site
perfusion can provide useful access to brain extracellular fluid when
suitable controls are incorporated for recovery variation and blood-brain
barrier seepage.
