AJP - Regu AJP: Gastrointestinal and Liver Physiology
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Am J Physiol Regul Integr Comp Physiol 234: R34-R38, 1978;
0363-6119/78 $5.00
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AJP - Regulatory, Integrative and Comparative Physiology, Vol 234, Issue 1 34-R38, Copyright © 1978 by American Physiological Society

ARTICLES

Studies of calcium-45 desaturation from kidney slices in flow-through chambers

T. Uchikawa and A. B. Borle

This paper describes a method to measure calcium fluxes and calcium exchangeable pools in tissue slices by continuous perifusion in flow-through chambers. 45Ca desaturation from rat kidney slices can be analyzed as in an open three-compartment catenary system. A set of equations is given to calculate all the relevant kinetic parameters from the triple exponential equations which best fit the desaturation curves. The results show that the kinetic parameters obtained in kidney slices by this new method are in the same order of magnitude as those previously observed in cultured monkey kidney cells.




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