AJP - Regulatory, Integrative and Comparative Physiology, Vol 233, Issue 3 83-R88, Copyright © 1977 by American Physiological Society
Evidence for enhanced uptake of ATP by liver and kidney in hemorrhagic shock
I. H. Chaudry, M. M. Sayeed and A. E. Baue
It has been shown that infusion of ATP-MgCl2 proved beneficial in the
treatment of shock; however, it is not known whether this effect is due to
improvement in the microcirculation or direct provision of energy or a
combination of the above or other effects. To elucidate the mechanism of
the salutary effect of ATP-MgCl2, we have now examined the in vitro uptake
of ATP by liver and kidney of animals in shock. Rats were bled to a mean
arterial pressure of 40 Torr and so maintained for 2 hrs. After the rats
were killed, liver and kidney were removed and slices of tissue (0.3-0.5 mm
thick) were incubated for 1 h in 1.0 ml of Krebs-HCO3 buffer containing 10
mM glucose, 5 mM MgCl2, and 5 mM [8-14C]ATP or 5 mM [8-14C]ADP, or 5 mM
[8-14C]AMP, or 5 mM [8-14C]adenosine in 95% O2-5% CO2 and then homogenized.
Tissue and medium samples were subjected to electrophoresis to separate and
measure the various nucleotides. The uptake of [14C]ATP but not that of
[14C]ADP or [14C]adenosine by liver and kidney slices from animals in shock
was 2.5 times greater than the corresponding uptake by control slices.
Thus, the beneficial effect of ATP-MgCl2 in shock could be due to provision
of energy directly to tissue in which ATP levels were lowered.
